Coding

Part:BBa_K258102

Designed by: Cihan Tastan   Group: iGEM09_METU-Gene   (2009-10-10)


PrtF domain of ABC transporter of Erwinia chrysanthemi

prtF, codes for a polypeptide of 462 amino acid residues (predicted Mr = 52.5 kd) that is probably initiated at an ATG codon located 15 bp downstream from the prtE gene stop codon TAA. It is preceded 11 bp upstream by a GGAAGG RBS consensus sequence.the PrtF protein is hydrophilic and analysis of the deduced amino acid sequence revealed the presence at its N-terminal end of a typical procaryotic signal peptide (italics in Figure 2) which contains three positively charged residues followed by a stretch of hydrophobic or neutral residues and ending at a consensus bacterial signal peptide cleavage site, Ala Gln Ala or Ala Met Gly.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 967
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1329
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None