![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K2581019
Designed by: Laura Sans Comerma Group: iGEM18_UPF_CRG_Barcelona (2018-10-09)
FadE gene with ATC inducible promoter with weak RBS.
The UPF_CRG_Barcelona iGEM team 2018 has created this part which consist in the expression of the acyl-coA dehydrogenase (fadE) gene which converts acyl-coA into enoyl-coA. This biobrick consists in the assembly of a double terminator which allows forward and reverse terminator (BBa_B0014), a tetR repressible promoter (BBa_R0040), a weak RBS (BBa_B0032) and the fadE gene obtained of the own E.Coli DH5-alpha strain genome (BBa_K2581014, being a twin of BBa_k861020, a part not available).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 768
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 551
Illegal SapI.rc site found at 1424
Illegal SapI.rc site found at 2567
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Categories
Parameters
None |