Intermediate

Part:BBa_K2581018

Designed by: Laura Sans Comerma   Group: iGEM18_UPF_CRG_Barcelona   (2018-10-09)


Intermediate fatty acid biosensor with sharp response

The UPF_CRG_Barcelona iGEM team 2018 has created this part as a intracellular biosensor of fatty acid uptake consisting in a high pass filter in order to reduce the high basal activity of the promoter in another efficient way. It consists of the assembly of a double terminator which allows forward and reverse terminator (BBa_B0014), a fatty acyl-CoA responsive promoter which expresses the downstream gene in the presence of fatty acids (BBa_k817002), a weak RBS (BBa_B0032), a CI repressor from E.coli phage lambda (+LVA) (BBa_C0051), again the double terminator (BBa_B0014), a modified lambda Prm promoter (BBa_ I12036), again the weak RBS (BBa_B0032), and an engineered mutant of red fluorescent protein from Discosoma striata (BBa_E1010).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 102
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1761
    Illegal AgeI site found at 1873
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None