Regulatory

Part:BBa_K2581013

Designed by: Laura Sans Comerma   Group: iGEM18_UPF_CRG_Barcelona   (2018-10-09)


pAR: Improved fatty acyl-CoA responsive promoter

The UPF_CRG_Barcelona iGEM team 2018 has create this part as an improved element from the existing fatty acid intracellular promoter pfadBA (Bba_k817002). This new promoter has two binding sites for fadR in comparison with the existing part. Therefore, we have demonstrate an enhanced function of this promoter resulting in a diminution of basility and hence, an improvement of this part.

This biobrick consists in the assembly of a double terminator which allows forward and reverse terminator (Bba_B0014), and the improved promoter based on the previous pfadBA DNA sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None