Composite

Part:BBa_K2581012

Designed by: Laura Sans Comerma   Group: iGEM18_UPF_CRG_Barcelona   (2018-10-09)


Improved fatty acid acyl-CoA biosensor with strong RBS

The UPF_CRG_Barcelona iGEM team 2018 has create this part as an improved element from the existing fatty acid intracellular promoter pfadBA (BBa_k817002). It consists of an intracellular biosensor of fatty acid uptake reducing the high basal activity that the mentioned promoter has. This biobrick consists in the assembly of a double terminator which allows forward and reverse terminator (BBa_B0014), our improved promoter based on the previous pfadBA DNA sequence (BBa_K2581013), an strong RBS based on Elowitz repressilator (BBa_B0034) and a reporter gene, an engineered mutant of red fluorescent protein from Discosoma striata (BBa_E1010).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 863
    Illegal AgeI site found at 975
  • 1000
    COMPATIBLE WITH RFC[1000]


IMPROVEMENT REFERENCE: Duesseldorf 2019

This part was improved with a better suited reporter gene in the composite part BBa_K2924019. In this improvement process, it was also characterized for its affinity to different lengths and concentrations of fatty acids.

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Categories
Parameters
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