Composite

Part:BBa_K2581005

Designed by: Laura Sans Comerma   Group: iGEM18_UPF_CRG_Barcelona   (2018-10-09)


Fatty acid acyl-CoA biosensor with weak RBS

The UPF_CRG_Barcelona iGEM team 2018 has created this part as a intracellular biosensor of fatty acid uptake. It consists of the assembly of a double terminator which allows forward and reverse terminator (Bba_B0014), a fatty acyl-CoA responsive promoter which expresses the downstream gene in the presence of fatty acids (Bba_k817002), a weak RBS (Bba_B0033) and a reporter gene, an engineered mutant of red fluorescent protein from Discosoma striata (Bba_E1010).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 102
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 755
    Illegal AgeI site found at 867
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None