Part:BBa_K2578820
Protein coding sequence for Homo sapiens metallothionein 2 (MT2A)
The part BBa_K2578820 is the composite part of the Homo sapiens metallothionein 2 synthesising gene (MT2A). The genomic sequence of the MT2A gene is referenced from NCBI (Gene ID 4502)[1].The MT1A gene, registered as BBa_K2578821, is designed to be the composite part BBa_K2578710 by adding consecutive promoter J23100[2], strong RBS B0032[3] and double terminator B0015[4].
Metallothioneins (MTs) are small cysteine-rich proteins made of 61-68 amino acids which can be found in a broad range of organisms, including both eukaryotes and prokaryotes. The MTs are expressed as intracellular protein. [5]
MTs are mainly responsible for metalloregulation in cells of living organisms. The rich Cys domains in MTs allow the non-covalent binding of trace metals such as cadmium, lead, copper and mercury, etc. [6,7]
MT2A has similar ability with MT1A gene. They are also involved in zinc homeostasis. But MT2A gene is found in liver, where MT1A was found in human kidney. The zinc binding ability of both gene are similar.[8]
We did not order the MT2A gene because we do not have enough time and we are out of budget of the 20kb range. Nonetheless, there may be a risk of cross species expression.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 389
Illegal BamHI site found at 190
Illegal BamHI site found at 597 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 541
- 1000COMPATIBLE WITH RFC[1000]
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