Protein coding sequence for Mouse metallothionein 2 (Mt2)
This is the basic part of the Mus musculus synthesising gene (Mt2). The genomic sequence of the Mt2 gene is referenced from NCBI (Gene ID 17750).
Metallothioneins are small cysteine-rich proteins made of 61-68 amino acids which can be found in a broad range of organisms, including both eukaryotes and prokaryotes. The metallothioneins are expressed as intracellular protein. 
Metallothioneins are mainly responsible for metalloregulation in cells of living organisms. The rich Cysteine domains in metallothioneins allow the non-covalent binding of trace metals such as cadmium(Cd2+), lead(Pb2+), copper(Cu1+), (Cu2+) and mercury(Hg2+), etc. [3,4]
The Mt2 gene has similar ability to Mt1 gene as they are equivalent proteins. They have strong binding affinity to heavy metals and serve severe function like metal homeostasis and metabolism and protect cell from oxidative stress of zinc ion in mouse liver[5,6]. But when compared to the metal binding ability to metal ions, results shown that Mt2 has better binding ability of Zinc ions (Zn2+) while Mt1 gene has better binding ability of copper ions(Cu1+) and cadmium ions (Cd2+).
The part is the gene of interest of the composite part BBa_K2578720.However, we failed to submit the part to the iGEM community as there are introns in the gene of interest. Therefore, there is an incompletion of the Mt2 subproject