Part:BBa_K2578710
Protein coding sequence for Mouse metallothionein 1 (Mt1)
The part BBa_K2578710 is the composite part of the Mus musculus metallothionein 1 synthesizing gene (Mt1). The genomic sequence of the Mt1 gene is referenced from NCBI (Gene ID 17748)[1]. The Mt1 gene, registered as BBa_K2578711, is designed to be the composite part BBa_K2578710 by adding consecutive promoter J23100[2], strong RBS B0032[3] and double terminator B0015[4].
Metallothioneins (MTs) are small cysteine-rich proteins made of 61-68 amino acids which can be found in a broad range of organisms, including both eukaryotes and prokaryotes. The MTs are expressed as intracellular protein. [5]
MTs are mainly responsible for metalloregulation in cells of living organisms. The rich Cys domains in MTs allow the non-covalent binding of trace metals such as cadmium, lead, copper and mercury, etc. [6,7]
The Mt1 gene has a strong binding affinity of Mt1 with heavy metals including copper, cadmium, mercury and zinc [8], has been proven. It was also shown that Mt1 serves functions as metal homeostasis, metabolism as well as protect cells from oxidative stress of zinc ion in mouse liver [8,9]. Therefore, it is highly possible that MT1 chelates heavy metal ions.
The designed part is synthesized by IDT, but the order failed as there are multiple restriction enzyme site and introns in the Mt1 gene BBa_K2578711. Therefore, there is an incompletion in the subproject on the Mt1 gene.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 438
Illegal PstI site found at 703 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal PstI site found at 703 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 282
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 438
Illegal PstI site found at 703 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 438
Illegal PstI site found at 703
Illegal AgeI site found at 316 - 1000COMPATIBLE WITH RFC[1000]
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