Part:BBa_K2578510
Protein coding sequence for Saccharomyces cerevisiae metallothionein (CUP1)
The part BBa_K2578510 is the composite part of the Saccharomyces cerevisiae metallothionein synthesizing gene (CUP1). The genomic sequence of the CUP1 gene is referenced from NCBI (Gene ID 856450)[1]. The CUP1 gene, registered as BBa_K2578511, is designed to be the composite part BBa_K2578510 by adding consecutive promoter J23100[2], strong RBS B0032[3] and double terminator B0015[4].
Metallothioneins (MTs) are small cysteine-rich proteins made of 61-68 amino acids which can be found in a broad range of organisms, including both eukaryotes and prokaryotes. The MTs are expressed as intracellular protein. [5] MTs are mainly responsible for metalloregulation in cells of living organisms. The rich Cys domains in MTs allow the non-covalent binding of trace metals such as cadmium, lead, copper and mercury, etc. [6,7]
Studies showed that the transcription of CUP1 is only induced by copper, instead of other heavy metals. [8] It was also shown that copper-binding property of yeast MT1 contributes to the metal detoxification in Saccharomyces cerevisiae and leads to its high tolerance in copper-contaminated environment [9]. Early studies have also demonstrated that CUP1 gene can be efficiently expressed in which made it a promising target of our study. [10]
The designed part is synthesized by IDT, but the order failed as IDT failed to synthesis the plasmid and insertion mutation occurs. Therefore, there is an incompletion in the subproject on the CUP1 gene.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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