Part:BBa_K2578410
Expression of mymT under constitutive promoter
The part BBa_K2578410[1] is the composite part of the Mycobacterium tuberculosis metallothionein synthesizing gene (mymT). The genomic sequence of the MymT gene is reference from Team Oxford in 2016. The MymT gene, registered by team Oxford in 2016 as BBa_K1980002, is designed by our teammates to be composite part BBa_K2578410 by adding consecutive promoter J23100[2], strong RBS B0032[3] and double terminator B0015[4].
Metallothioneins (MTs) are small cysteine-rich proteins made of 61-68 amino acids which can be found in a broad range of organisms, including both eukaryotes and prokaryotes. The MTs are expressed as intracellular protein. [5]
MTs are mainly responsible for metalloregulation in cells of living organisms. The rich Cys domains in MTs allow the non-covalent binding of trace metals such as cadmium, lead, copper and mercury, etc. [6,7]
The plasmid containing composite part BBa_K2578410 is ordered and synthesized by IDT. The plasmid is also cloned into pSB1C3 backbone for part submission.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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