Part:BBa_K257021

PLacI + RBS-TetR + Ter + Ptet + RFP

This part can be used to introduce a delay between induction and expression of a desired gene.

It is another possibility for our delay system test. The first part of this delay test is PBad+RBS-LacI-LVA + RBS + YFP + Ter.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 1495
  Illegal AgeI site found at 1607
  
• 1000
  COMPATIBLE WITH RFC[1000]

Functional Parameters

n/a	PLacI + RBS-TetR + Ter + Ptet + RFP

Measurement of BBa_K257021 delay

BBa_K257021 was grown overnight in LB medium with:
- Glucose 1 %
- nothing
- IPTG (0.2mg/ml)

Each of the 3 overnight cultures was then diluted to the 1/100 in each of the 3 conditions. Fluorescence and OD were assessed in a Tecan plate reader.

In the second graph, fluorescence was normalized by the mean Fluo/OD of all the conditions where mRFP1 is continuously expressed.

We observe a delay of ~10000sec between the beginning of the experiment and the changes in RFP expression.

Note that the delay is shorter for the LB + glucose -> LB + IPTG shift than for the LB + glucose -> LB shift.