Signalling
FecA*

Part:BBa_K257019

Designed by: Romain Bodinier   Group: iGEM09_Paris   (2009-10-19)

FecA mutated - constitutive activity of the fec operon


The fec transduction pathway is unique because it allows a signal to be passed from the extracellular milieu down to the cytoplasm, thus being transmitted through both the outer and inner membrane. Briefly, the outermembrane protein FecA binds ferric citrate, change its conformation to interact with FecR which is an transmembrane protein located in the inner membrane. Then FecR is able to activate FecI an alternative sigma factor specific to Pfec.

In some variant of the system, the activated FecR homologues would repress FecI activity instead of activating it.

We designed FecA to have a constitutive activity.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1290
    Illegal BglII site found at 2203
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 89


[edit]
Categories
Parameters
None