Composite
clyA_RFP

Part:BBa_K257010

Designed by: Vicard Du   Group: iGEM09_Paris   (2009-06-20)

clyA + RFP

Insertion of the protein fusion clyA-RFP in the outer membrane of the cell. During vesiculation process, we can observe vesicles by fluorescence.

Safety Note

ClyA (hlyE) is a toxin. For more information see here: BBa_K257002

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 84
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1494
    Illegal AgeI site found at 1606
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None