Part:BBa_K257007

ClyA_Fusion_N-Term

ClyA (or HlyE) is an alpha-PFT for Pore Forming Toxins.

PFTs are potent virulence factors class starting in a soluble form to an outer membrane-integrated pore. They exhibit their toxic effect either by membrane permeability barrier destruction or by toxic components delivery through the pores which forming by several assembly 8 or 13 ClyA subunits.

ClyA can be used to co-localize fully functional heterologous proteins directly in bacterial OMVs
We can fuse GFP to the C or N term of Cly A, to track OMVs easily.
ClyA is capable of co-localizing a variety of structurally diverse fusion partners to the surface of E. coli and their released vesicles, but only when the periplasmic disulfide bond-forming machinery was present ,it’s makes OMVs an ideal structure to transport hydrophobic compounds like membrane proteins into the host.
Cly A confers vesicle binding to and invasion of host cells.
ClyA was significantly enriched in OMVs relative to other lumenal and membrane bound OMV proteins.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 84
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

References

The structure of a cytolytic a-helical toxin pore reveals its assembly mechanism, M.Mueller & N.Ban. 2009 - [http://www.ncbi.nlm.nih.gov/pubmed/19421192 | 19421192 ]
S.N. Wai, B.Lindmark, T.Soderblom, A.Takade, M.Westermark, J.Oscarsson, et al. Vesicle-mediated export and assembly of pore-forming oligomers of the enterobacterial ClyA cytotoxin, 2003, Cell, 115, 25–35. [http://www.ncbi.nlm.nih.gov/pubmed/14532000 14532000]
F.J del Castillo, F. Moreno. and I.del Castillo. Secretion of the Escherichia coli K-12 SheA hemolysin is independent of its cytolytic activity, 2001, FEMS Microbiol.Lett. 204, 281–285. [http://www.ncbi.nlm.nih.gov/pubmed/11731136 11731136]
J.E.Galen, L.Zhao, M.Chinchilla, J.Y.Wang,M.F.Pasetti, J.Green and M.M. Levine. Adaptation of the endogenous Salmonella enterica serovar Typhi clyA-encoded hemolysin for antigen export enhances the immunogenicity of anthrax protective antigen domain 4 expressed by the attenuated live-vector vaccine strain CVD 908-htrA, 2004, Infect. Immun. 72, 7096–7106.[http://www.ncbi.nlm.nih.gov/pubmed/15557633 15557633]
J.Y. Kim, A.M. Doody, D. J. Chen, G.H. Cremona, M.L. Shuler, D.Putnam,and M.P. DeLisa.Engineered. Bacterial Outer Membrane Vesicles with Enhanced Functionality, 2008, J. Mol. Biol. 380, 51–66. [http://www.ncbi.nlm.nih.gov/pubmed/18511069 18511069]
N.C.Kesty, M.J.Kuehn. Incorporation of heterologous outer membrane and periplasmic proteins into Escherichia coli outer membrane vesicles, 2004, J Biol Chem. 279(3):2069-76.[http://www.ncbi.nlm.nih.gov/pubmed/14578354 14578354]

[1] The structure of a cytolytic a-helical toxin pore reveals its assembly mechanism, M.Mueller & N.Ban. 2009 - [http://www.ncbi.nlm.nih.gov/pubmed/19421192 | 19421192 ]

[2] S.N. Wai, B.Lindmark, T.Soderblom, A.Takade, M.Westermark, J.Oscarsson, et al. Vesicle-mediated export and assembly of pore-forming oligomers of the enterobacterial ClyA cytotoxin, 2003, Cell, 115, 25–35. [http://www.ncbi.nlm.nih.gov/pubmed/14532000 14532000]

[3] F.J del Castillo, F. Moreno. and I.del Castillo. Secretion of the Escherichia coli K-12 SheA hemolysin is independent of its cytolytic activity, 2001, FEMS Microbiol.Lett. 204, 281–285. [http://www.ncbi.nlm.nih.gov/pubmed/11731136 11731136]

[4] J.E.Galen, L.Zhao, M.Chinchilla, J.Y.Wang,M.F.Pasetti, J.Green and M.M. Levine. Adaptation of the endogenous Salmonella enterica serovar Typhi clyA-encoded hemolysin for antigen export enhances the immunogenicity of anthrax protective antigen domain 4 expressed by the attenuated live-vector vaccine strain CVD 908-htrA, 2004, Infect. Immun. 72, 7096–7106.[http://www.ncbi.nlm.nih.gov/pubmed/15557633 15557633]

[5] J.Y. Kim, A.M. Doody, D. J. Chen, G.H. Cremona, M.L. Shuler, D.Putnam,and M.P. DeLisa.Engineered. Bacterial Outer Membrane Vesicles with Enhanced Functionality, 2008, J. Mol. Biol. 380, 51–66. [http://www.ncbi.nlm.nih.gov/pubmed/18511069 18511069]

[6] N.C.Kesty, M.J.Kuehn. Incorporation of heterologous outer membrane and periplasmic proteins into Escherichia coli outer membrane vesicles, 2004, J Biol Chem. 279(3):2069-76.[http://www.ncbi.nlm.nih.gov/pubmed/14578354 14578354]