Part:BBa_K2570017

Application of PLA: dsrA+lacI+PlacI+hns+ProU+TyrB+LDH+rocG

In our project, we combined temperature promoter dsrABBa_K2570015 and salt-concentration promoter proU BBa_K2467001 as a salt-concentration and temperature control promoter to reach the aim of producing antibacterial, PLA. The temperature range of dsrA is from 25 degree to 42 degree. With temperature increase, the promote efficiency is getting higher. [1]The salt-concentration promoter proU provides sensitivity to the salt concentration to human skin. proU is a promoter existed in E.coli, induced by low salt concentration. And the H-NS BBa_K2570016 is a global-regulated protein which could bind to genome and specifically inhibit proU.[2] When temperature reached 37 degree, suitable for human body, temperature promoter dsrA will express protein lacI. The promoter induced by lacI(PlacI) will be inhibited, the global regulate protein H-NS will not be express. Then, people sweat, the salt in the sweat will decide the production of PLA .

On the other hand, when our circuits in the container, which does not start to work. Not enough room temperature to induce dsrA, the temperature promoter. The promoter induced by LacI will express global regulate protein H-NS, which could repress proU. Our lotion is in a salt-free container which could prevent producing PLA at room temperature

Application scenario: When people sweat, there is a specific salt concentration and temperature on the skin. Only two conditions are both satisfied, the PLA will be released to kill the bacteria under the axilla.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1587
  Illegal BglII site found at 5419
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 6204
  Illegal NgoMIV site found at 6743
  Illegal AgeI site found at 4480
  Illegal AgeI site found at 5892
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 3324
  Illegal SapI.rc site found at 154
  Illegal SapI.rc site found at 1973