Part:BBa_K2570015
dsrA
Temperature is one environmental variable that all organisms must deal with. Besides the direct effect of temperature on enzymatic reactions, temperature response involves remodeling of the expression pattern of genes, affecting transcription, RNA stability, translation efficiency, and/or proteolysis. RpoS is a stationary-phase and stress response sigma factor in E. coli and many other bacteria. One environmental cue that increases RpoS synthesis is low temperature (below 37°C). This increase is completely dependent upon dsrA, a small noncoding RNA[2]. dsrA was shown to stimulate RpoS translation by pairing with a portion of the mRNA upstream of the RpoS translation start that can pair with and occlude the ribosome-binding site of the transcript [3]. dsrA is more abundant in E. coli at low growth temperatures than at higher temperatures, resulting in the increased RpoS expression at low temperatures [4]. Temperature affects both the synthesis and the stability of dsrA, leading to thermocontrol of RpoS translation. In our project, we try to control our circuits express antibacterial, PLA only at the time people sweat. There is a specific salt concentration and different temperature between people skin and normal temperature. Thus, we combined temperature promoter dsrA and salt-concentration promoter proU BBa_K2467001 as a salt-concentration and temperature control composite part BBa_K2570011 to realize the aim of producing antibacterial, PLA BBa_K2570018.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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