Part:BBa_K2570010

Production of 2-PE: Aro10+PAR+TyrB

We used the tnaA gene deletion strain of Escherichia coli as an engineering strain, and co-overexpressing the aromatic transaminase from E. coli (TyrB)BBa_K2570002, phenyl pyruvate decarboxylase from S. cerevisiae (Aro10)BBa_K2570001, PAR BBa_K2570000 the phenyla-cetaldehyde reductase from the rose (Rosa sp.) under the control of the constitutive promoter BBa_J23100. The phenylalanine is converted to phenyl pyruvate by transamination, then acetaldehyde is formed by decarboxylation, and finally 2-phenylethyl is produced by dehydrogenation. The 2-PE biosynthetic pathway was constructed, and finally the metabolic pathway and optimization of the catalytic conditions were carried out to significantly increase the yield of 2-PE.

Construction and optimization of the 2-PE biosynthetic pathway in E.coli. The overall stoichio- metric equation of the reactions in this biosynthetic system is:

L-Phe+2-OG+NAD(P)H+H+→2-PE+L-Glu+NAD(P)++CO2

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  Illegal NheI site found at 2242
  Illegal NotI site found at 2605
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 4044
  
• 1000
  COMPATIBLE WITH RFC[1000]