Coding

Part:BBa_K2570008

Designed by: Luying He   Group: iGEM18_FJNU-China   (2018-09-30)


adhE2

This sequence encodes for adhE2, which is the third enzyme responsible for degrading unpleasant axilla odors E-3-methyl-3-hexenoic acid (E-3M2H).It could be used after atoD and atoA for further conversion of the acid to the alcohol. These works are in conjunctions with BBa_K2570006 and BBa_K2570007， aiming to eliminate unpleasant axilla odors E-3M2H by converting the odorant acids into alcohols.

Fig.1 The structure of adhE2.

The adhE2 sequence was obtained by PCR.

Fig.1 PCR result of adhE2.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 916
    Illegal AgeI site found at 169
    Illegal AgeI site found at 571
    Illegal AgeI site found at 1008
    Illegal AgeI site found at 1942
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None