Coding

Part:BBa_K2570001

Designed by: Luying He   Group: iGEM18_FJNU-China   (2018-09-13)


Aro10

The phenylpyruvate decarboxylase from S. cerevisiae (Aro10, NP 010668.3). The phenylpyruvate is converted to acetaldehyde by decarboxylation.

A heterologous Ehrlich pathway was constructed for 2-PE bio-synthesis in E. coli by co-overexpressing the aromatic transaminase from E. coli (TyrB, WP032305522.1)BBa_K2570002, phenylpyruvate decarboxylase from S. cerevisiae (Aro10, NP 010668.3) and phenylcetaldehyde reductase from the rose (Par , BAG13450.2 )BBa_K2570000 under the control of the constitutive promoter BBa_J23100. The resulting E.coli strain BW25113 pSB1C3-Aro10-PAR-TyrB was used as the whole-cell biocatalyst, and 2-PE was obtained from 50mM L-Phe in a 12 h bioconversion .

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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