Part:BBa_K257000

fec promoter (Pfec) - inducible through the Fec transduction pathway (ferric citrate)

Inducible promoter controled by the FecARI pathway.

Usage and Biology

The fec transduction pathway is unique because it allows a signal to be passed from the extracellular milieu down to the cytoplasm, thus being transmitted through both the outer and inner membrane. Briefly, the outermembrane protein FecA binds ferric citrate, change its conformation to interact with FecR which is an transmembrane protein located in the inner membrane. Then FecR is able to activate FecI an alternative sigma factor specific to Pfec.

In some variant of the system, the activated FecR homologues would repress FecI activity instead of activating it.

For more information, see: Gene regulation by transmembrane signaling. Volkmar Braun et al., 2006

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Functional Parameters

See BBa_K257000 caracterisation in BBa_K257023