Coding
CHE

Part:BBa_K256023

Designed by: Zhu Meng   Group: iGEM09_NTU-Singapore   (2009-10-17)

Cholesteryl Esterase (CHE)

From Pseudomonas aeruginosa; Code for expression of cholesteryl esterase (CHE) which convert cholesteryl ester to free cholesterol and fatty acid.


Usage and Biology

CHE catalyzes the hydration reactions of CEs into free cholesterol and fatty acids with high specificity.

Cholesteryl Ester + H2O → cholesterol + RCOOH


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 702
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
familyHydrolase
functionHydrolyze cholesteryl esters into free cholesterol and fatty acid
originPseudomonas Aeruginosa