Coding

Part:BBa_K2557049

Designed by: Ge JT   Group: iGEM18_NAU-CHINA   (2018-10-09)


TP901-RDF

TP901-RDF is a fusion protein of TP901 recombinase and the corresponding RDF. This fusion protein recognizes the site produced by TP901 recombination and flips the sequence back.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1590
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 1590
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 348
    Illegal BglII site found at 649
    Illegal BglII site found at 1243
    Illegal BglII site found at 1369
    Illegal BglII site found at 1596
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1590
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1590
    Illegal AgeI site found at 6
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 846
    Illegal SapI.rc site found at 1790


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Categories
Parameters
None