Coding

Part:BBa_K2557048

Designed by: Ge JT   Group: iGEM18_NAU-CHINA   (2018-10-09)


PhiC31-RDF

PhiC31-RDF is a fusion protein of PhiC31 recombinase and the corresponding RDF. This fusion protein recognizes the site produced by PhiC31 recombination and flips the sequence back.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 911
    Illegal BamHI site found at 997
    Illegal BamHI site found at 1244
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 78
    Illegal NgoMIV site found at 307
    Illegal NgoMIV site found at 349
    Illegal NgoMIV site found at 1920
    Illegal AgeI site found at 6
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 603
    Illegal BsaI.rc site found at 1006
    Illegal BsaI.rc site found at 1359
    Illegal BsaI.rc site found at 2001
    Illegal SapI.rc site found at 2603


[edit]
Categories
Parameters
None