DNA

Part:BBa_K2557032

Designed by: Sheng XU   Group: iGEM18_NAU-CHINA   (2018-10-09)


miniCMV promoter

minimal cytomegalovirus promoter. The minimal CMV (miniCMV) promoter is a low expression synthetic promoter in mammalian cells.

Characterization

Fig. 1 Fluorescence microscope observation of HEK 293T only transfect with plasmids containing promoters with tetO sequence.

Fig. 1 shows that the strength of the three promoters in HEK 293T cells is from strong to weak: Ubc > EF1 α > miniCMV.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/eukaryote
Parameters
None