Composite

Part:BBa_K2557027

Designed by: Ge JT   Group: iGEM18_NAU-CHINA   (2018-10-10)


Ubc promoter--TP901-RDF

According to the feedback of digital model, the recombination efficiency and expression level of recombinase-RDF will affect the stability of the switch we designed. Therefore, we need to characterize the promoters of different intensities and RDF, and hope to get an optimal combination scheme. The Ubc promoter and TP901-RDF are characterized here.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1998
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 1998
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 756
    Illegal BglII site found at 1057
    Illegal BglII site found at 1651
    Illegal BglII site found at 1777
    Illegal BglII site found at 2004
    Illegal BamHI site found at 408
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1998
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1998
    Illegal AgeI site found at 414
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1254
    Illegal SapI.rc site found at 2198


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Parameters
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