Composite

Part:BBa_K2557026

Designed by: Ge JT   Group: iGEM18_NAU-CHINA   (2018-10-10)


EF1α promoter--TP901-RDF

According to the feedback of digital model, the recombination efficiency and expression level of recombinase-RDF will affect the stability of the switch we designed. Therefore, we need to characterize the promoters of different intensities and RDF, and hope to get an optimal combination scheme. The EF1α promoter and TP901-RDF are characterized here.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1810
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 1810
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 568
    Illegal BglII site found at 869
    Illegal BglII site found at 1463
    Illegal BglII site found at 1589
    Illegal BglII site found at 1816
    Illegal BamHI site found at 220
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1810
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1810
    Illegal AgeI site found at 226
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1066
    Illegal SapI.rc site found at 2010


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Categories
Parameters
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