Composite

Part:BBa_K2557021

Designed by: Ge JT   Group: iGEM18_NAU-CHINA   (2018-10-10)


Ubc promoter-Bxb1-RDF

According to the feedback of digital model, the recombination efficiency and expression level of recombinase-RDF will affect the stability of the switch we designed. Therefore, we need to characterize the promoters of different intensities and RDF, and hope to get an optimal combination scheme. The Ubc promoter and Bxb1-RDF are characterized here.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1974
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 1974
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1182
    Illegal BglII site found at 1980
    Illegal BamHI site found at 408
    Illegal BamHI site found at 623
    Illegal BamHI site found at 939
    Illegal BamHI site found at 1647
    Illegal BamHI site found at 1938
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1974
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1974
    Illegal NgoMIV site found at 924
    Illegal NgoMIV site found at 2457
    Illegal NgoMIV site found at 2640
    Illegal AgeI site found at 414
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 644


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Categories
Parameters
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