Composite

Part:BBa_K2557020

Designed by: Ge JT   Group: iGEM18_NAU-CHINA   (2018-10-10)


EF1α promoter-Bxb1-RDF

According to the feedback of digital model, the recombination efficiency and expression level of recombinase-RDF will affect the stability of the switch we designed. Therefore, we need to characterize the promoters of different intensities and RDF, and hope to get an optimal combination scheme. The EF1α promoter and Bxb1-RDF are characterized here.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1786
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 1786
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 994
    Illegal BglII site found at 1792
    Illegal BamHI site found at 220
    Illegal BamHI site found at 435
    Illegal BamHI site found at 751
    Illegal BamHI site found at 1459
    Illegal BamHI site found at 1750
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1786
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1786
    Illegal NgoMIV site found at 736
    Illegal NgoMIV site found at 2269
    Illegal NgoMIV site found at 2452
    Illegal AgeI site found at 226
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 456


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Categories
Parameters
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