Composite

Part:BBa_K2557019

Designed by: Ge JT   Group: iGEM18_NAU-CHINA   (2018-10-10)


miniCMV promoter-Bxb1-RDF

According to the feedback of digital model, the recombination efficiency and expression level of recombinase-RDF will affect the stability of the switch we designed. Therefore, we need to characterize the promoters of different intensities and RDF, and hope to get an optimal combination scheme. The miniCMV promoter and Bxb1-RDF are characterized here.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1612
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 1612
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 820
    Illegal BglII site found at 1618
    Illegal BamHI site found at 46
    Illegal BamHI site found at 261
    Illegal BamHI site found at 577
    Illegal BamHI site found at 1285
    Illegal BamHI site found at 1576
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1612
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1612
    Illegal NgoMIV site found at 562
    Illegal NgoMIV site found at 2095
    Illegal NgoMIV site found at 2278
    Illegal AgeI site found at 52
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 282


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Categories
Parameters
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