Composite

Part:BBa_K2557018

Designed by: Ge JT   Group: iGEM18_NAU-CHINA   (2018-10-09)


TetO-Ubc promoter-TP901

The TetO sequence, SV40 NLS, flag tag, Ubc promoter, and TP901 recombinase are ligated in sequence, and participate in intracellular signal processing. According to the feedback of the digital model, the different strength promoters upstream of the recombinase and the recombination efficiency of the recombinase will affect the stability of the system. Therefore, in order to optimize our system, we used three promoters and three recombinases, hoping to find the optimal solution.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 753
    Illegal PstI site found at 1326
    Illegal PstI site found at 2037
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1326
    Illegal PstI site found at 2037
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1348
    Illegal BamHI site found at 687
    Illegal BamHI site found at 2292
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 753
    Illegal PstI site found at 1326
    Illegal PstI site found at 2037
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 753
    Illegal PstI site found at 1326
    Illegal PstI site found at 2037
    Illegal NgoMIV site found at 792
    Illegal NgoMIV site found at 936
    Illegal AgeI site found at 693
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1545


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