Composite

Part:BBa_K2557015

Designed by: Ge JT   Group: iGEM18_NAU-CHINA   (2018-10-09)


TetO-Ubc promoter-PhiC31

The TetO sequence, SV40 NLS, flag tag, Ubc promoter, and PhiC31 recombinase are ligated in sequence, and participate in intracellular signal processing. According to the feedback of the digital model, the different strength promoters upstream of the recombinase and the recombination efficiency of the recombinase will affect the stability of the system. Therefore, in order to optimize our system, we used three promoters and three recombinases, hoping to find the optimal solution.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 754
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 687
    Illegal BamHI site found at 1605
    Illegal BamHI site found at 1691
    Illegal BamHI site found at 1938
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 754
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 754
    Illegal NgoMIV site found at 772
    Illegal NgoMIV site found at 1001
    Illegal NgoMIV site found at 1043
    Illegal AgeI site found at 693
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1297
    Illegal BsaI.rc site found at 1700
    Illegal BsaI.rc site found at 2053


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Parameters
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