Composite

Part:BBa_K2557014

Designed by: Ge JT   Group: iGEM18_NAU-CHINA   (2018-10-09)


TetO-EF1α-PhiC31

The TetO sequence, SV40 NLS, flag tag, EF1α promoter, and PhiC31 recombinase are ligated in sequence, and participate in intracellular signal processing. According to the feedback of the digital model, the different strength promoters upstream of the recombinase and the recombination efficiency of the recombinase will affect the stability of the system. Therefore, in order to optimize our system, we used three promoters and three recombinases, hoping to find the optimal solution.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 566
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 499
    Illegal BamHI site found at 1417
    Illegal BamHI site found at 1503
    Illegal BamHI site found at 1750
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 566
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 566
    Illegal NgoMIV site found at 584
    Illegal NgoMIV site found at 813
    Illegal NgoMIV site found at 855
    Illegal AgeI site found at 505
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1109
    Illegal BsaI.rc site found at 1512
    Illegal BsaI.rc site found at 1865


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Categories
Parameters
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