Composite

Part:BBa_K2557013

Designed by: Ge JT   Group: iGEM18_NAU-CHINA   (2018-10-09)


TetO-miniCMV-PhiC31

The TetO sequence, SV40 NLS, flag tag, minCMV promoter, and PhiC31 recombinase are ligated in sequence, and participate in intracellular signal processing.According to the feedback of the digital model, the different strength promoters upstream of the recombinase and the recombination efficiency of the recombinase will affect the stability of the system. Therefore, in order to optimize our system, we used three promoters and three recombinases, hoping to find the optimal solution.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 392
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 325
    Illegal BamHI site found at 1243
    Illegal BamHI site found at 1329
    Illegal BamHI site found at 1576
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 392
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 392
    Illegal NgoMIV site found at 410
    Illegal NgoMIV site found at 639
    Illegal NgoMIV site found at 681
    Illegal AgeI site found at 331
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 935
    Illegal BsaI.rc site found at 1338
    Illegal BsaI.rc site found at 1691


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Parameters
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