Composite

Part:BBa_K2557012

Designed by: Ge JT   Group: iGEM18_NAU-CHINA   (2018-10-09)


TetO-Ubc promoter-Bxb1

The TetO sequence, SV40 NLS, flag tag, Ubc promoter, and Bxb1 recombinase are ligated in sequence, and participate in intracellular signal processing.According to the feedback of the digital model, the different strength promoters upstream of the recombinase and the recombination efficiency of the recombinase will affect the stability of the system. Therefore, in order to optimize our system, we used three promoters and three recombinases, hoping to find the optimal solution.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 753
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1467
    Illegal BamHI site found at 687
    Illegal BamHI site found at 908
    Illegal BamHI site found at 1224
    Illegal BamHI site found at 1932
    Illegal BamHI site found at 2223
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 753
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 753
    Illegal NgoMIV site found at 1209
    Illegal AgeI site found at 693
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 929


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Categories
Parameters
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