RNA

Part:BBa_K2556041

Designed by: Yankang Wu   Group: iGEM18_ZJUT-China   (2018-10-04)


sgRNA-panD

sgRNA is an artificial RNA which is designed to bind a certain DNA sequence. It contains the 20-bp complementary region (N20) with the requisite NGG PAM matching genomic loci of interest and the sequence of tracrRNA. It will guide the nuclease Cas protein 9(Cas9) to the target. It is of great importance in CRISPR/Cas9 system. We designed the sgRNA which specifically targets the gene panD in order to demonstrate our CRISPR/Cas9 system.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None