Part:BBa_K2525019

pLac+RBS+PhlF+T

This part contains pLac(R0010), RBS (B0032), PhlF (K2525016) and Term (K1725081). The PhlF repressor has higher cooperativity which negatively regulates promoter pPhlF. The high cooperativity of this part is of great significance to synthetic biology, as it can be used to give steeper response curves for repression. Ideally, when we tend to look at genetic elements as digital logic tools, we require steep transitions of protein levels from 0 to 1 or 1 to 0 (representing an ON and OFF state). However, due to various limitations in biological systems, the response is often in the form of a gradient and not a steep curve. The part can be regulated with LacI repressor and IPTG induction in a system where LacI is present.

Note: This part is the same as BBa_K2525007. However, that part is in a Kanamycin resistance backbone (pSB1K3), while this is in a Chloramphenicol backbone (pSB1C3)

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]