Part:BBa_K2525003
Orf2 Repressor (High Cooperativity, n = 6.1)
This part contains the coding sequence for the Orf2 repressor, which is known to have very high cooperativity. It was first reported by Voigt et al (Nature Chem Biol, 2016), and characterized by conversion into a not gate, by placing the Orf2 gene under the pTac promoter. This promoter is inducible by IPTG, and produced increasing levels of Orf2 upon increments of IPTG. This was then connected to a node containing the YFP gene under an Orf2 repressible promoter. This created a NOT Gate with IPTG as the input, and the input - output curve was plotted to determine the cooperativity, which is 6.1.
The high cooperativity of this part is of great significance to synthetic biology, as it can be used to give steeper response curves for repression. Ideally, when we tend to look at genetic elements as digital logic tools, we require steep transitions of protein levels from 0 to 1 or 1 to 0 (representing and ON and OFF state). However, due to various limitations in biological systems, the response is often in the form of a gradient and not a steep curve. Due to its high cooperativity, Orf2 can give a much better and steeper curve than most traditional repressors. This is highly desirable.
At high levels, Orf2 is toxic to E.coli.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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