Composite

Part:BBa_K2525000

Designed by: Abhilash Patel   Group: iGEM17_IIT_Delhi   (2017-10-27)


pOrf2-RBS-GFP-T

pORF2 is a repressible promoter ORF2 repressor. It is derived from Streptomyces griseus. The Orf2 repressor hase a high cooperativity, of 6.1 (Stanton et al, 2008). A higher cooperativity of such a sort gives rise to steeper response compared to repressor tetR. Such behavior can be used for digitized dynamical circuits. The promoter is combined with RBS-GFP-T to give orf2 repressible gfp expression to use in genetic circuits.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 41
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 726


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Parameters
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