Composite

Part:BBa_K2522001

Designed by: Dallas McDonald   Group: iGEM17_US_AFRL_CarrollHS   (2017-10-03)


sfGFP produced in presence of 3OC12

The plasmid is engineered to detect communication signals between the pathogenic E. coli strains. The first plasmid created with PLas_sfGFP promoter is activated by 3OC12 in order to produce Green Fluorescent Protein (GFP) PLas promoter detects 3OC12, a known quorum sensing molecule. The team used this plasmid as an initial experimentation using promoter sites and the observations of positive responses to quorum sensing molecules.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1006
    Illegal NheI site found at 1029
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1382
    Illegal AgeI site found at 1579
  • 1000
    COMPATIBLE WITH RFC[1000]


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