Regulatory

Part:BBa_K2500004

Designed by: Irma Telarovic   Group: iGEM17_ETH_Zurich   (2017-10-24)


TlpA: Temperature-Dependent Transcriptional Repressor

TlpA is a constitutively expressed protein, which binds to promoter pTlpA and blocks the start of transcription of genes downstream of pTlpA.1 The binding to the promoter is reversibly deactivated by temperatures above 37 °C with full deactivation at 45 °C. Parameters influencing the final expression of the genes downstream of pTlpA are the constitutive promotor and RBS of TlpA itself and the RBS of the pTlpA downstream genes. By tweaking these sequences, it should be possible to obtain a wide range of leakiness and fold changes (fold-change up to 200-fold).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 793
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1096
  • 1000
    COMPATIBLE WITH RFC[1000]


References

1. Piraner, Dan I., et al. "Tunable thermal bioswitches for in vivo control of microbial therapeutics." Nature chemical biology 13.1 (2017): 75-80.

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