Composite

Part:BBa_K2497999

Designed by: Catrin Schuler   Group: iGEM17_Stuttgart   (2017-10-27)


KerP from Pseudomonas aeruginosa

Keratinases are proteolytic enzymes that are applied in agro-industrial, pharmaceutical and biomedicals fields. In our project we used keratinases for establishing a microbial tube cleaner. Hair mostly consists of alpha-keratin which is hydrolyzed by keratinases. By using the keratinases we want to avoid chemical compounds that are recently present in tube cleaners. Many different keratinases produced by different microorganisms (e.g. Bacillus subtilis) have been reported. These enzymes vary in their biochemical and biophysical properties e.g. temperature and pH activity range. Biobrick part BBa_K2497999 contains a pBAD promotor (BBa_K206000) composite with kerP gene from Pseudomonas aeruginosa. Gene sequence was optimized to eliminate illegal restriction sites.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1160
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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