Part:BBa_K2495001
Device expressing the putative O-antigen transporter under control of an arabinose inducible promote
Putative O-antigen transporter encoding sequence under the control of an arabinose inducible promoter and constitutive promoter. It is used to code for the O-antigen transporter characteristic of E.coli O157:H7 strain that produces Shiga-toxin.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1205
Illegal NheI site found at 1225
Illegal NheI site found at 1248 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1144
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2108
Illegal SapI site found at 961
Illegal SapI site found at 1443
Experimental Validation
Throughout our project, we successfully ligated our gene rfbE to the pSB1CR linearized backbone and transformed it into competent DH5-alpha E. coli cells. The part was validated by running PCR on the gene fragment using primers BBa_K2495019 and BBa_K2495020. These primers amplified a region of approximately 1000 bp. Appropriate bands were seen in Lane 3, which indicated that the part was successfully ligated and transformed.
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