Composite

Part:BBa_K2495001

Designed by: Adrienne Chang   Group: iGEM17_NYU_Abu_Dhabi   (2017-10-09)


Device expressing the putative O-antigen transporter under control of an arabinose inducible promote

Putative O-antigen transporter encoding sequence under the control of an arabinose inducible promoter and constitutive promoter. It is used to code for the O-antigen transporter characteristic of E.coli O157:H7 strain that produces Shiga-toxin.

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
    Illegal NheI site found at 1225
    Illegal NheI site found at 1248
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2108

Experimental Validation

Throughout our project, we successfully ligated our gene rfbE to the pSB1CR linearized backbone and transformed it into competent DH5-alpha E. coli cells. The part was validated by running PCR on the gene fragment using primers BBa_K2495019 and BBa_K2495020. These primers amplified a region of approximately 1000 bp. Appropriate bands were seen in Lane 3, which indicated that the part was successfully ligated and transformed.

"NYUAD_pSB1C3_rfbE_validation.png"


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