Device expressing the putative O-antigen transporter under control of an arabinose inducible promote
Putative O-antigen transporter encoding sequence under the control of an arabinose inducible promoter and constitutive promoter. It is used to code for the O-antigen transporter characteristic of E.coli O157:H7 strain that produces Shiga-toxin.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12Illegal NheI site found at 1205
Illegal NheI site found at 1225
Illegal NheI site found at 1248
- 21Illegal BamHI site found at 1144
- 23COMPATIBLE WITH RFC
- 25Illegal AgeI site found at 979
- 1000Illegal BsaI site found at 2108
Throughout our project, we successfully ligated our gene rfbE to the pSB1CR linearized backbone and transformed it into competent DH5-alpha E. coli cells. The part was validated by running PCR on the gene fragment using primers BBa_K2495019 and BBa_K2495020. These primers amplified a region of approximately 1000 bp. Appropriate bands were seen in Lane 3, which indicated that the part was successfully ligated and transformed.