Part:BBa_K2492000

GFP (optimised, for use in the C. elegans chassis)

This part arises from BBa_K309016[1], which is improved and characterized by our group. Modified GFP with three introns which can help well expression of GFP in C.elegans. In our group, we use it to indicate the location of neuron AWB in C.elegans. We integrated str1::CoCHR::GEM-GECO::GFP transgene in C.elegans by microinjection. Modified GFP will be highly expressed in AWB driven by promoter str1(no biobrick part).

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 264
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 797
  

Test for GFP: localize the neuron-AWB We successfully ligated the str-1::Chrimson::GEM-GECO::GFP and injected the plasmids into C.elegans through microinjection. This picture was one of our stable inheritance strains. We used 488nm to excite AWB and observe at 500-540nm wavelength through confocal. We successfully observed a single green slender neuron on the anterior region of C.elegans. Comparing to the normal localisation of AWB, we indicated that the fluoresecence of GFP appeared in AWB. Thus we successfully used GFP to localise the position of target neuron.

GFP expressed in AWB