Composite

Part:BBa_K2481109

Designed by: Erin Kelly   Group: iGEM17_Lethbridge_HS   (2017-10-27)


indC expression construct

The IndC Indigoidine Synthetase from Photorhabdus luminescens laumondii TT01 is a nonribosomal peptide synthetase (NRPS) which produces the blue pigment indigoidine from two glutamine molecules. This expression construct allows for the IPTG inducuple expression of IndC in E. coli BL21(DE3).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1311
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2574


[edit]
Categories
Parameters
None