Part:BBa_K2481107
crtY and crtZ expression construct
crtY and crtZ are both members of the carotenoid biosynthesis pathway. crtY (lycopene cyclase) from Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087. Converts lycopene to beta-carotene. crtZ (beta-carotene hydroxylase) from Pantoea ananatis. Converts beta-carotene to the yellow pigment zeaxnathin. The crtY-crtZ parts, containing RBSs native to Pantoea ananatis had been previously developed by Chris French of the iGEM07_Edinburgh group (2007-10-24, parts BBa_I742155 and BBa_I742158). These had previously been expressed under constitutive promoters such as J23103 but not under a T7 promoter that can be used in E. coli BL21(DE3). We kept the native RBSs on these genes and incorporated it into a new part under the control of the T7 promoter (BBa_I712074) and the terminator BBa_B0015. As with the melA expression construct descried above, these new control elements will allow users to specifically control when expression starts through an IPTG induction system, as well as limit leaky expression.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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