Composite

Part:BBa_K2481106

Designed by: Erin Kelly   Group: iGEM17_Lethbridge_HS   (2017-10-27)


indB expression construct

indB is a putative phosphatase from Streptomyces crhomofuscus whose native function has not been well characterized. It has been shown that expression of IndB in conjuction with the indogoidine sythase indC will increase indigoidine yields. This part allows for the ITPG inducible epxression of IndB in E. coli BL21(DE3).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 701
    Illegal BamHI site found at 852
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1135
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None