Composite

Part:BBa_K2477010

Designed by: Paula Kleyman, Thea Ornstein, Yuzhu Shi, Joyce Song   Group: iGEM17_UMaryland   (2017-10-26)


TLP Secretion System

This circuit constitutively expresses the antifungal agent, TLP, tagged with a 6x His-tag, a 2x GS linker, and an HlyA secretion peptide. The HlyA tag allows for secretion using the HlyB/HlyD system. Both HlyB and HlyD are also encoded on this part and are under control of an arabinose promoter. Please note that there is no scar site between the end of TLP (the GSGS linker) and the start of the HlyA tag.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 2180
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2119
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 181
    Illegal AgeI site found at 284
    Illegal AgeI site found at 326
    Illegal AgeI site found at 1954
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 468
    Illegal SapI site found at 1936


[edit]
Categories
Parameters
None