Coding

Part:BBa_K2477004

Designed by: Jacob Premo   Group: iGEM17_UMaryland   (2017-10-26)


Cas9

This is the Cas9 gene that was utilized in our experiments. It is capable of cleaving specific DNA sequences when expressed alongside an sgRNA. This part was taken from addgene plasmid pML107.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1078
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 169
    Illegal SapI.rc site found at 1159


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Parameters
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