Composite
SDM GFP

Part:BBa_K2477003

Designed by: Jacob Premo   Group: iGEM17_UMaryland   (2017-10-25)


GFP with PAM site (BBa_K2477000) expression system

This part constituitively expresses GFP. The synonymous mutation introducing a PAM site in the GFP gene allows for the interaction of this part with an sgRNA overlapping the chromophore region of GFP (BBa_K2477001). This allows for selection against non-mutants in this overlap region when expressed alongside the sgRNA and a Cas9 nuclease.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 705


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Categories
Parameters
None